Orange tabby cat long hair2/9/2024 ![]() ![]() Their agile movements and lightning-fast reflexes make them excellent hunters of toys and bring joy to anyone who witnesses their playful endeavors. Orange tabbies are often seen engaging in playful antics, chasing toys, or even playing fetch with their favorite humans. Their inquisitive nature often leads them to investigate every nook and cranny, making their environment their personal playground. These kitties have a natural curiosity and love to explore their surroundings. If you’re looking for a fun and playful feline companion, an orange tabby cat might be the perfect choice. While orange tabbies are generally known for their affectionate nature, each cat is unique and may exhibit their own quirks and preferences. However, it’s important to remember that individual differences in personality can still vary from cat to cat. It’s fascinating to think that their coat color and personality might be intertwined, creating a cat that is not only visually striking but also incredibly loving. ![]() Some researchers propose that the same gene that determines the orange color is linked to certain personality traits, including increased sociability and friendliness. Studies suggest that the gene responsible for the orange coat color might also influence their behavior. Whether it’s snuggling up on the couch or curling up in bed, these kitties love to be close to their loved ones. They often form strong bonds with their human companions, seeking out warmth and companionship. One of the most endearing qualities of orange tabby cats is their affectionate nature. What Makes Orange Tabbies So Affectionate? Let’s dive deeper into what makes orange tabbies so affectionate and playful. These feline companions have a special charm that captivates the hearts of many. Orange tabby cats are not only known for their striking coat color, but also for their unique personality and temperament. Read More The Ultimate Guide to Cat Urinary Health: Top Supplements You Need to Know Unraveling the Personality and Temperament of Orange Tabbies Whether you find yourself drawn to their intricate stripes or mesmerized by their vibrant shades of orange, there is no denying the allure and charm of these extraordinary feline companions. Orange tabby cats, with their unique coat patterns and captivating genetics, continue to amaze and enchant cat enthusiasts worldwide. So, if you ever come across a female orange tabby, consider yourself fortunate to witness the beauty and rarity that she represents. While female orange tabbies do exist, they are a minority compared to their male counterparts. This genetic quirk not only explains the higher prevalence of male orange tabbies but also sheds light on the rarity of their female counterparts. On the other hand, females need two orange genes to display the same color. The gene responsible for the orange color is located on the X chromosome, which means that male cats only need one orange gene to express the captivating orange hue in their coats. ![]() Have you ever wondered why orange tabby cats are predominantly male? The answer lies in their genetics. Unveiling the Genetics Behind Orange Tabby Cats These distinct variations make orange tabbies even more special, as no two cats are exactly alike in their appearance. Some display deep reddish tones, resembling the warmth of a glowing sunset, while others boast bright and vibrant hues that catch the eye from afar. Not only do their coat patterns differ, but orange tabby cats can also have different shades of orange. From the classic mackerel tabby with its narrow stripes resembling fish skeletons, to the elegant and bold blotched tabby, these cats never fail to impress with their diverse and stunning appearances. One of the most fascinating aspects of orange tabby cats is the wide range of coat patterns they exhibit. Exploring Different Types of Orange Tabby Cats These delightful feline beauties come in various shades and patterns, each showcasing their own distinct charm. Orange tabby cats, with their striking coats and unique characteristics, have captivated the hearts of cat lovers around the world. The Fascinating World of Orange Tabby Cats In this ultimate guide, we will dive deep into the world of orange tabbies, uncovering intriguing facts, exploring their lifespan, and shedding light on their intelligence. But orange tabbies are more than just a pretty face – they possess fascinating traits, genetics, and personalities that set them apart from other cats. With their vibrant orange fur and unique patterns, they never fail to catch the eye and warm the hearts of those who encounter them. Orange tabby cats have a captivating charm that has made them one of the most popular and beloved cat breeds. ![]()
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![]() Sign in to your account security dashboard. Tap the three vertical dots at the top right, and then tap "Settings. Download & install the Microsoft Authenticator app to your mobile device. However, this article uses the Microsoft Authenticator app. You aren't required to use the Microsoft Authenticator app, and you can select a different app during the set up process. Step 1: Go to your Microsoft account profile configuration page: Step 2: Connect to your account using the authentication methods currently available to you (if you are configuring your Azure account, you can request a Temporary Access Pass - TAP - to your administrator) Step 4: Select the option Security Key and. To do this, open Microsoft Authenticator. You can set up an authenticator app to send a notification to your mobile device or to send you a verification code as your security verification method. If you need to recover your accounts on a new phone, you'll have to turn on the backup option on your old one. In this final phase of deploying high availability federated authentication for Microsoft 365 in Azure infrastructure services, you get and install a certificate issued by a public certification authority, verify your configuration, and then install and run Microsoft Entra Connect on the directory synchronization server. Turn on the Backup Option on Your Old Phone It provides simple push notifications so the user does not have to enter codes. You'll then be able to use those if you experience any issues when trying to recover your accounts. Microsoft authenticator is the preferred solution for approving MFA requests. Make sure you have a copy of the backup codes for each account before you attempt to change your authenticator device. Most accounts provide backup codes you can use if you've lost or damaged your phone. Note that 2FA is designed to make it extremely hard to access an account unless you have the 2FA code. Authenticator app: Works in scenarios where Microsoft Entra authentication is used, including across all browsers, during Windows 10 setup, and with integrated mobile apps on any operating system. You have to re-create your 2FA accounts on your new phone manually.įortunately, Microsoft Authenticator provides a backup and recovery option. We found that there's no way to export all your accounts, and then import them onto a new phone. ![]() ![]() Previously, we looked at moving 2FA accounts in Google Authenticator to a new phone. Using an authenticator app for two-factor authentication (2FA) is more secure than SMS messages, but what if you switch phones? Here's how to move your 2FA accounts if you use Microsoft Authenticator. Set up your Microsoft 365 sign-in for multi-factor authentication Sign in to Microsoft 365 with your work or school account with your password like you. ![]() Calculator to subtract dates2/9/2024 ![]() ![]() How to return a date based on values in other cells To display a date rather than a serial number, apply the desired Date format to the formula cell. For instance, combine the YEAR and TODAY to get a serial number for the first day of the current year.Īnd this formula outputs a serial number for the first day of the current month in the current year: Instead of specifying the values representing the year, month and day directly in a formula, you can have some or all arguments driven by of other Excel date functions. ![]() This is the most obvious use of the DATE function in Excel.įor example, to return a serial number corresponding to 2, use this formula: DATE formula to return a serial number for a date Excel DATE formula examplesīelow you will find a few examples of using DATE formulas in Excel beginning with the simplest ones. Tip. At first sight, supplying negative values in the month or day argument of the Excel DATE function may seem absurd, but in practice it may turn out quite useful, for example in the complex formula that converts a week number to a date. If the year argument is less than 0 or greater than 9999, a DATE formula will return the #NUM! error.If the year argument is between inclusive, Excel adds the specified number to 1900.If the year argument is between 19 inclusive, Excel uses exactly the value you supplied to create a date. ![]() For more details, please see Excel date format. By default, Microsoft Excel for Windows uses the 1900 system where Januis represented by the serial number 1. YearĮxcel interprets the year argument according to the date system set up on your computer. In practice, there may be some unobvious pitfalls that the below tips will help you avoid. The DATE syntax looks crystal clear and straightforward on the surface. The DATE function is available in all versions of Excel 365 - Excel 2007. Month - an integer representing the month of the year, from 1 (January) to 12 (December).ĭay - an integer corresponding to the day of the month, from 1 to 31. ![]() Dewalt to ridgid battery adapter2/9/2024 ![]() ![]() A steel can/filter of a coffee machine looked like someone had hit it with a hammer. You're spot on.Ĭargo planes have crashed, cargo planes performed emergency landings because of "funky stuff" happening with LiIon batteries/cells on board.Ī long time ago, long before those crashes, someone I briefly knew posted pictures of the aftermath when a lithium ion battery had exploded in his kitchen while charging. DeWalt does have a lot compatibility within their family, some of the chargers can accept both their 12 volt and their 20 volt packs, the 60 v packs shown in the video clip can be used on 20v tools and the chargers take both (60 and 20) batteries. Given the cost of my Festool stuff, I'm not inclined to experiment with it. Otherwise there is a lot of risk of blowing up the batteries. They don't document any of that, so mixing successfully requires a lot of tearing things apart, and a lot of knowledge of what all the stuff you uncover is. While adapting is theoretically possible, and does work in limited cases, some manufacturers put the battery control circuitry in the tool, while others put it in the battery pack, and no doubt, some others do something in-between. ![]() Now I have 2 Festool, 2 Bosch, and still have the DeWalt. Up to a year ago, I had two battery/charger types, DeWalt and Ryobi, I retired all the Ryobi stuff, but in the meantime I added some new. YOU are the circuitry between the tool and the battery and you need to make sure you aren’t killing either or going to burn down your shop.Build a charging center to organize them. You can do what you are asking, but you need to be careful. Dewalt doesn’t want to trust that Ryobi is going to do the monitoring correctly and doesn’t want to be responsible when somebody smokes their tools or batteries. This is why there is no battery standard. Some low draw tools don’t even listen to the battery because they can’t draw enough current to damage it. What does happen is the battery has electronics inside the cell, anything from a thermistor to a microprocessor monitoring all the cells that will communicate with only that brands tools and tell the tool to stop running if there is a problem. You can basially arc weld with the batteries and they will keep giving current until you either melt down the li-ion cells or melt through the wires or contacts. There is no battery that I know of that doesn’t connect the cells directly to the terminals – except for Flexvolt which has a mechanical selector switch for 20V or 60V. (And even in that case, you might confuse the coulomb-counter in the board, if present. Of course do NOT use these adapters for charging! Always use the native charger, unless you have your own balance charger and know exactly what you’re doing. It’s still prudent not to run something all the way until it stops – when you hear the tool slowing down, stop right there and recharge the battery! (The 12’s not so much!), and of course the NiCd don’t care what you do, so I consider it safe to intermix every which way. I believe all the 18v lithium batteries have a protection board in 'em. I’ve gutted the cheap Ryobi battery-cap chargers (the P111 and there’s one other whose number I don’t know) and the P150 battery-gauge, to bring out the power contacts to run stuff like my TS100 soldering iron, a series of LED lights, and yes, another gutted DeWalt 18v battery so we could run my buddy’s DeWalt radio off my spare Ryobi batteries. It moved the center of gravity back a bit too, which was welcome on an otherwise-nose-heavy tool. I made a simple adapter by chopping up a scrap Ryobi flashlight to get the battery socket, and grafting it into a gutted Black&Decker battery, to allow my dad’s B&D weedwacker to run from his Ryobi batteries. ![]() ![]() All the parts are on Thingiverse, just gotta combine 'em and print one… I wish SOMEone would make an adapter to use Ridgid 18v slide batteries on Ryobi post tools. Of course these work in other Ryobi 18v tools, too. Surebonder makes a hot-glue gun that takes Ryobi 18v post batteries, and then they make adapters for Makita and Milwaukee batteries to fit their glue guns. ![]() Sakura clicker hentai patch2/9/2024 ![]() You can now start Sakura Clicker from Steam as normal! Have fun. Your game folder should now look like this:Ĭongratulations! You have now successfully installed the nude mod for Sakura Clicker. Don’t worry anymore since Let’s talk about Gamers brings you the trick to activate the best hentai mod for Sakura Clicker for over 18 years. Let the patcher do his thing and wait until it closes. Drag and drop the extracted files (the files inside the extracted folders) from ‘patchv1.3’ and ‘quickbms’ into the game folder.Ĥ. Paste them into the original ‘Sakura Clicker’ folder and delete the recently created one to prevent auto update confusion with Steam.ĩ. Cut out all files in the created folder.Ĩ. Go into the recently created folder and rename ‘Tap Dungeon’ to ‘Sakura Clicker’.ħ. Rename the original ‘Sakura Clicker’ to ‘Sakura Clicker Backup’ if you want to backup you data.Ħ. Click ‘Extract here’ (this will create a new folder named ‘Sakura Clicker’)ĥ. This uncensor patch unlocks everything removed in the Steam version of the Sakura Dungeon game. The patch includes: 24+ CGs, extar events and level of clothing damage. Locate your Steam games folder (most likely ‘C:\Program Files (x86)\Steam\SteamApps\common\Sakura Clicker) or by right clicking on ‘Sakura Clicker’ in your Steam library > Properties > Local files > Browse local files.Ĥ. This uncensor patch unlocks everything removed in the Steam version of the Sakura Dungeon game. ![]() As far as the stories go, they're all their own separate stories and. I may have gotten a couple of titles mixed up, but that's the general order. Find the file assets.rpa in the folder Game. The release order for the Sakura series goes like this: Sakura Spirit > Sakura Angels > Sakura Fantasy > Sakura Beach > Sakura Swim Club > Sakura Beach 2 > Sakura Santa. Download the game and go to the folder Game. Copy assets.rpa file with replacement to folder Game Enjoy ( ) 1. ![]() you can download it and copy to the game save file. Actual nude mod (click ‘Lastest version of patch’) (includes image material)Įxtract ‘quickbms’ and ‘patchv1.3’ by right clicking > Extract here (this will create a folder out of the. Sakura Succubus 3: R18 Patch Guide (Uncensor Patch) Here is the R18 patch for Sakura Succubus 3 game. QuickBMS (direct download) (file importer)ģ. Favorite Share Created by Requiered components You'll only need WinRar if you haven't already. WinRar (to create/extract a copy of Sakura Clicker)Ģ. You’ll only need WinRar if you haven’t already.ġ. Who else wants a hentai mod for Sakura Clicker, but can’t get a good tutorial? Nothing works because other tutorials are inaccurate? Here’s the trick! How to Enable 18+ Content ![]() Imazing license code reddit2/9/2024 ![]() It's been great for a few years, the only way i'm going to furhter support iMazing is if they figure out a way to bring back wifi backups without the iOS nag (unlock) screen and change their licensing model to something that doesn't hold me hostage from new device upgrades. but i'll be happy the day that i come across the next solution that replaces this one. this is the kind of thing that will undoubtedly gather interest to a cheaper or community supported alternative. find a better way to sell products, cause changing your model to limit functionality to N devices per purchase is just greedy. and since iOS 16.1's breaking change with wifi backups as is, I may just go back to USB backups in Finder when the time comes.īeing limited to this app running on two computers made sense from a licensing perspective. And downloading a copy of my ipa for backup and preservasion is done via itune app management.įor whom exactly, here I am glade to pay for an upgrade to imazing3 if only we didn’t have to put up with the horrible device lock issue.Īgreed, i'll be very disappointedly looking for alternative solutions for when an updated macOS version inevitably stops iMazing V2 from working. Which I have not found a work around for, but again I need to instal old ipa unavailable from the AppStore only on old devices which still support those apps and games. ![]() The best feature and the most noticing one is the ability to install IPA. With iPad os and iOS moving closer and closer to a more accessible in game and create apps piggy backing on the file app, I have been accessing my files for ages without imazing. ![]() Whilst I would require update for new os versions, imazing is a necessity for older devices rather than newer ones when it comes to spyware detection.Īpp management, well this was useful back when I used to really be into iOS gaming, and all. As well device do change over the shears and there is a lot of faster which will necessite acquiring a new license in a less convenient time.Īs it stands right now I am debating wether I should upgrade or move back to itune, because of this confusion. Honnestly I would rather pay for unlimited devices than per devices. If the license still validated my original agreement and allow me to upgrade to 3 and use the set devices ( 5) I am of with it. ![]() Can people who upgraded to imazing 3 keep both imazing legacy and 3? ![]() Edit diablo ii bin file2/8/2024 ![]() I'll try and post a compiled version when I clean up some attachment space.ĭiablo_II_Simple_Name_Changer.Fix several bugs and extend some files based on the original v1.13c TXT. Refreash ( ) EndFunc Func Refreash ( ) $file = GUICtrlRead ( $txtFilePath ) if $file = "" then Return $filehandle = FileOpen ( $file, 4 ) $size = FileGetSize ( $file ) dim $byteA for $i = 0 to $size - 1 $byteA = FileRead ( $filehandle, 1 ) Next $name = "" for $i = 20 to 35 $name &= chr ( Dec ( Hex ( Binary ( $byteA ) ) ) ) Next GUICtrlSetData ( $txtCharName, $name ) EndFunc func FixCheckSum ( ) dim $checksum $byteA = 0x00 $byteA = 0x00 $byteA = 0x00 $byteA = 0x00 $boolCarry = 0 for $i = 0 to UBound ( $byteA ) - 1 $temp = Dec ( Hex ( Binary ( $byteA ) ) ) + $boolCarry $checksum = $checksum * 2 + $temp $checksum = $checksum * 2 if $checksum > 255 Then $checksum = $checksum + ( $checksum - Mod ( $checksum, 256 ) ) / 256 $checksum = Mod ( $checksum, 256 ) EndIf $checksum = $checksum * 2 if $checksum > 255 Then $checksum = $checksum + ( $checksum - Mod ( $checksum, 256 ) ) / 256 $checksum = Mod ( $checksum, 256 ) EndIf $checksum = $checksum * 2 if $checksum > 255 Then $checksum = $checksum + ( $checksum - Mod ( $checksum, 256 ) ) / 256 $checksum = Mod ( $checksum, 256 ) EndIf If $checksum > 255 Then $checksum = Mod ( $checksum, 256 ) EndIf if BitAND ( $checksum, 0x80 ) 0 Then $boolCarry = 1 Else $boolCarry = 0 EndIf Next $byteA = "0x" & Hex ( $checksum, 2 ) $byteA = "0x" & Hex ( $checksum, 2 ) $byteA = "0x" & Hex ( $checksum, 2 ) $byteA = "0x" & Hex ( $checksum, 2 ) EndFunc WriteFile ( ) MsgBox ( 0, "Done", "File Saved" ) EndFunc Func WriteFile ( ) $Path = GUICtrlRead ( $txtFilePath ) $Dir = StringMid ( $Path, 1, StringInStr ( $Path, "\", 0 ,- 1 ) ) $newName = GUICtrlRead ( $txtNewName ) $OutFile = FileOpen ( $Dir & $NewName & ".d2s", 18 ) for $i = 0 to UBound ( $byteA ) - 1 FileWrite ( $OutFile, $byteA ) Next FileClose ( $OutFile ) EndFunc func Close ( ) Exit EndFunc func Browse ( ) $Path = FileOpenDialog ( "Select Save File", , "Diablo II Save Files(*.d2s)" ) if $Path = "" then Return GUICtrlSetData ( $txtFilePath, $Path ) ![]() key or map files, so you have to do that manually if you want them" ) EndFunc func Apply ( ) $newName = GUICtrlRead ( $txtNewName ) if $newName = "" or StringLen ( $newName ) 16 Then MsgBox ( 0, "Error", "Invalid Name" ) Return EndIf for $i = 20 to 35 $byteA = Binary ( "0x" & Hex ( Asc ( StringMid ( $newName, $i - 19, 1 ) ), 2 ) ) Next It's quite entertaining for a refreashing Diablo II experiance, and it is the inspiration for this script.Įxpand collapse popup Opt ( "GuiOnEventMode", 1 ) GuiCreate ( "Diablo II - Simple Name Changer", 392, 323 ) GUISetOnEvent ( - 3, "Close" ) $txtFilePath = GuiCtrlCreateInput ( "", 90, 20, 200, 20 ) GuiCtrlCreateLabel ( "Current Name:", 10, 60, 70, 20 ) $txtCharName = GuiCtrlCreateInput ( "", 90, 60, 200, 20 ) GUICtrlSetState ( - 1, 128 ) GuiCtrlCreateLabel ( "File:", 40, 20, 40, 20 ) $btnBrowse = GuiCtrlCreateButton ( "Browse", 310, 20, 60, 20 ) GUICtrlSetOnEvent ( - 1, "Browse" ) $btnRefreash = GuiCtrlCreateButton ( "Refreash", 310, 60, 60, 20 ) GUICtrlSetOnEvent ( - 1, "Refreash" ) GuiCtrlCreateLabel ( "New Name", 10, 150, 60, 20 ) $txtNewName = GuiCtrlCreateInput ( "", 90, 150, 200, 20 ) $btnBrwose = GuiCtrlCreateButton ( "Apply", 310, 150, 60, 20 ) GUICtrlSetOnEvent ( - 1, "Apply" ) $btnClose = GuiCtrlCreateButton ( "Close", 300, 290, 90, 30 ) GUICtrlSetOnEvent ( - 3, "Close" ) $btnAbout = GuiCtrlCreateButton ( "About", 0, 290, 70, 30 ) GUICtrlSetOnEvent ( - 1, "About" ) Global $byteA GuiSetState ( ) While 1 Sleep ( 100 ) WEnd func About ( ) MsgBox ( 0, "Simple Name Changer V1.0 by Evilertoaster", "This is designed to rename a single player character for Daiblo II 1.10 and 1.11. d2s files) and don't try to do anything fancy.Īlso, as a side note, I would like to recomend the following mod to any Diablo II fans. This script only looks at the file header and only write to the name section.Īlso keep in mind there is no elaborate checking for errors, invalid files or other circumstancial use. As a result, they fail to work on save files for mod'ed versions of Diablo II. They provide anaylisis and automatic fixing of the save file, but for certian cases this is not ideal (namely, when using a MOD that has non-stnadard save files). It was written becuase most other utilities that can do name changes are 'too' functional. It is indended for use in Diablo II LOD 1.10 and 1.11. Select a save file, pick a new name, and hit apply. ![]() Here's a script to change your characters name in Diablo II. ![]() Wedding dinner emcee script2/8/2024 ![]() While it may not be in the script, it is also the emcee’s task to inform guests of important information, such as the direction of the comfort rooms and the opening and closing hours of the bar.“Good evening, everyone, and welcome to Jamie and Jim’s wedding reception!” If this is observed, it is introduced and presided over by the emcee. Observing Western Customs – Many wedding receptions in Singapore now include Western traditions, such as the bouquet/garter throw. On behalf of the couple and their families, the emcee thanks everyone once again for attending the reception and wishes them a pleasant evening ahead. If someone will perform at this part, the emcee will introduce him/her.Įnd of the Ceremony – The emcee concludes the event. ![]() Photo by Zonzon Productions Picture-Taking with the Couple – The emcee announces that the couple will now go through each table to have their pictures taken with the guests. It is the emcee’s duty to give a short but heartfelt reply to the couple’s messages and assist them if they become too emotional. The Newlyweds’ Speech – With everyone is seated and the party has taken a more serious mood, the emcee asks the bride and groom to give their speech or special messages to the guests. Everyone is asked to take their seats after this. Yam Seng – A unique feature to Singapore weddings is the yam seng, where the emcee asks the yam seng party to come to the stage and make the three toasts for the couple (for eternal love, for a lifetime together, and for many children). Opening of the Champagne – Everyone remains standing as the emcee directs to couple to come on stage and open a bottle of champagne in preparation for the toast. ![]() He/she will then ask everyone to stand up once again and welcome the couple on their second march in. Second March In – The emcee announces the end of the meal and wishes that everyone have enjoyed the food. For Singapore weddings, this usually includes the couple’s preparation and the gatecrash ceremony earlier in the day. Second Photo Montage – As the last dish or the dessert is being served, the emcee once again directs everyone attention to the screen, which will show another photo montage or a video clip. ![]() A joke or two will be welcome at this time to put everyone at ease. Photo by Affinity Pictures Announcement of Dinner – The emcee informs the party that food will be served shortly and wishes that they enjoy what the hosts have prepared for them. It’s recommended that the emcee say something about the importance of this ritual as a symbol of marriage. The emcee should take special care to pronounce everyone’s names properly.įirst Photo Montage – The emcee directs the guests’ attention to a screen monitor, which will show a photo montage of the couple that shows them in their younger years.įirsts March In – Having set the right mood for excitement, the emcee requests everyone to stand up and cheer to welcome the newlywed for their first march in.Ĭake Cutting Ceremony – Once the couple has made their way to the stage, the emcee thanks them for the show and invites them to cut the wedding cake. He/she then introduces him/herself, acknowledges the presence of important guests (parents of the couple, sponsors, etc.). Introduction – The emcee greets the audience, welcomes them to the couple’s wedding reception, and thanks them for coming. Invitation to be Seated – At the appointed time, the emcee requests all the attendees to take their seats as the program is about to begin. While there is no fixed template for an emcee script, this guide includes the most commonly observed sections of a Chinese/non-religious type of wedding reception in Singapore. A master of the ceremony or emcee serves as the conductor of the wedding reception, the one responsible for directing the sequence of events of the program. ![]() ![]() The error bars present the mean ± SEM (* p < 0.05).Ĭathepsin B/L genetic depletion in MEF cells causes accumulation of cholesterol and lysosomal proteins. ATP-Binding Cassette sub-family A member 1 (ABCA1) and Niemann-Pick C1 protein (NPC1) mRNA levels presented as a fold change. ![]() (D) RT-PCR Expression of the cholesterol egress genes in the control, PADK, U18666A- and Leu/NH 4Cl-treated SH-SY5Y cells, normalized to β-actin and quantified by 2−ΔΔCt method using control sample as calibrator. Error bars present the mean ± standard deviation (*** p < 0.001). Student t-test was used for statistical analysis. (C) Quantification of Western blot results of the 3 independent experiments was performed by ImageJ. (B) Western blot of SH-SY5Y control and PADK treated cells using LC3, ABCA1 and NPC1 antibody. Cholesterol (filipin staining, white) and NPC1 (green). (A) Confocal microscopy of SH-SY5Y control and PADK treated cells. Error bars present the mean ± standard deviation (** p < 0.01, *** p < 0.001).Ĭathepsin B/L inhibition causes NPC disease-like cholesterol accumulation in SH-SY5Y cells. (B) Western blot of CHOwt cells treated with different inhibitors using ABCA1, NPC1 and NPC2 antibody. (A) Confocal microscopy of CHOwt cells treated with different inhibitors. Error bars present the mean ± standard deviation (* p < 0.05, ** p < 0.01).Ĭathepsin B/L inhibition causes NPC disease-like cholesterol accumulation in CHO cells. ![]() (E) Quantification of Western blot results of the 3 independent experiments was performed by ImageJ. (D) Western blot of CHOwt cells treated with different inhibitors and CHO NPC1-null cells using LC3 antibody. (C) Confocal microscopy of CHOwt cells treated with different inhibitors and CHO NPC1-null cells. Error bars present the mean ± standard deviation (* p < 0.05, ** p < 0.01). (B) Quantification of Western blot results of the 3 independent experiments was performed by ImageJ. Western blot analysis of EGFR in SH-SY5Y cells treated with different inhibitors at different time points. Inhibition of cathepsin B/L and not cathepsin D causes lysosomal dysfunction. ![]() Kindergarten 2 game move save file2/8/2024 ![]() Danner will enter the classroom and will be confused about how you got in. Go to the top right of the classroom and go to the book. ![]() Go to the air vent in the bottom right of the bathroom and unscrew it. Say: She's not lying! > That does sound crazy. Jerome will be angry at Lily and will place one of the strange devices on the floor in front of Lily. Knock on the stall farthest to the right. Exit the cafeteria and go up the stairs to the girl's bathroom. The lunch lady will leave to tell the principal about the food fight. Then give Buggs the burger and he will start a food fight by throwing a burger at Nugget. Say okay and then buy a burger from Margaret the Lunch Lady. Jerome will give Buggs his burgers and Buggs says he needs one more. Go to Buggs and ask if he would want to start a food fight. Danner then sends everyone down to lunch and Carla and Jerome will come to you. Go up to Jerome and say: She'll be in the bathroom during lunch.ĭr. Talk to Carla and say: They'll be in the girl's bathroom. Stevie will then see you and send you up to class. Not wanting to go there, she goes back up. Carla will protest and Stevie threatens to send her to the principals office. Stevie will tell Carla to go back to class and give her study hall. When you get inside, Carla will come down the stairs and ask Stevie if you are still in the bathroom. Go back through the grate and into the school. Say: Who are you guys? > Jerome is after Lily. Then, Lily will come out of the box and tell Billy to come out. Go over to the box furthest to the right and squeak the plushie. There should be a room with four cardboard boxes in it, two stacked and two separate beside it. Go outside with Carla, then progress down the stairs and unlock the red door on the far right to go outside to the handicap ramp. Take the assessment and grab a prize, it doesn't matter which one. ![]() > No problem.Ĭarla will run over and ask if the kid you rescued was Nugget. Go to the grate, and tell Nugget: I'm gonna get you out of there. > Could it be them? > What are our options? > Okay then. > Okay then.īefore talking to Nugget, go over to Carla and talk to her. it was on the news? > Find a foster family? > Who do you think did this? > She's not even here. Go over to Jerome and offer him the tissue, then respond to him as follows: Why? What's wrong? > Yes. > How does the laser help? > There was a lot of stuff. Select the following options: Do you want this? > Finally what? > What are you talking about? > Which is? > Okay. When you are ready, start the day and talk to Carla. Things That Go Boom is a mission in Kindergarten 2 (game) Mission Walkthrough Īt the start of the day when you are in your bedroom, you need to select the Monstermon Plushie ( If you can Dodge a Nugget), A+ ( Flowers for Diana) and Penny's Laser Beam ( Opposites Attract) ![]() AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |